MichaelÌýNagel
Mechanisms of transcription re-initiation
Transcription reinitiation occurs following a pioneering round of transcription but remains poorly understood. Reinitiation events produce most RNA transcripts and are essential for robust biological responses.ÌýÌýReinitiation may occur through mechanisms distinct from "first-round" transcription initiation.ÌýÌýA reinitiation scaffold, discovered inÌýS. cerevisiaeÌýby the Steve Hahn lab, consists of a set of general transcription factors that remain promoter-associated following the first round of transcription; this scaffold may similarly operate at human genes, to facilitate transcription re-initiation.ÌýÌýMy project implements biochemical and cell-based methods to characterize theÌýmolecular mechanisms that control transcription re-initiation in human cells.Ìý
Publications
Nagel, M; Taatjes, DJ. Regulation of RNA polymerase II transcription through re-initiation and bursting. Mol Cell 2025, 85: 1907-1919.
Luyties, O; Sanford, L; Rodino, J; Nagel, M; Jones, T; Rimel, JK; Ebmeier, CC; Palacio, M; Shelby, GS; Cozzolino, K; Brennan, F; Hartzog, A; Saucedo, MB; Watts, LP; Spencer, S; Kugel, JF; Dowell, RD; Taatjes DJ. Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends. Cell Rep 2025, 44: 115904.Ìý
Bassett, J; Rimel, JK; Basu, S; Basnet, P; Luo, J; Engel, KL; Nagel, MT; Woyciehowsky, A; Ebmeier, CC; Kaplan, CD; Taatjes, DJ; Ranish, JA. Systematic mutagenesis of TFIIH subunit p52/Tfb2 identifies residues required for XPB/Ssl2 subunit function and genetic interactions with TFB6. J Biol ChemÌý2022, 298: 102433.ÌýÌý
Zhou, W., E. Ramos, X. Zhu, P. M. Fisher, M. E. Kidane, B. H. Vanderloop, C. D. Thomas, J. Yan, U. Singha, M. Chaudhuri, M. T. Nagel, and W. D. Nes. Steroidal antibiotics are antimetabolites of Acanthamoeba steroidogenesis with phylogenetic implications.ÌýJÌýLipid ResÌý2019, 60: 981–994.